منابع مشابه
Genome engineering in Bacillus anthracis using tyrosine site-specific recombinases
Tyrosine site-specific recombinases (T-SSR) are polynucleotidyltransferases that catalyze cutting and joining reactions between short specific DNA sequences. We developed three systems for performing genetic modifications in Bacillus anthracis that use T-SSR and their cognate target sequences, namely Escherichia coli bacteriophage P1 Cre-loxP, Saccharomyces cerevisiae Flp-FRT, and a newly disco...
متن کاملPhylogenetic analysis of the streptococcal tyrosine recombinases Tyrosine recombinase protein sequences were obtained from BLASTp searches
Supporting Materials and Methods pGh9-derivative plasmids and E. coli strains constructions pGh9-derivative plasmids were constructed as follows: relevant pairs of oligonucleotides containing XmaI linkers were annealed together, generating DNA duplexes with two XmaI-compatible ends. Only the left end of the duplexes recreated a full XmaI site, allowing further determination of its orientation b...
متن کاملThe XerC recombinase of Proteus mirabilis: characterization and interaction with other tyrosine recombinases.
XerC and XerD are two site-specific recombinases, which act on different sites to maintain replicons in a monomeric state. This system, which was first discovered and studied in Escherichia coli, is present in several species including Proteus mirabilis, where the XerD recombinase was previously characterized by our laboratory. In this paper, we report the presence of the xerC gene in P. mirabi...
متن کاملThe integrase family of tyrosine recombinases: evolution of a conserved active site domain.
The integrases are a diverse family of tyrosine recombinases which rearrange DNA duplexes by means of conservative site-specific recombination reactions. Members of this family, of which the well-studied lambda Int protein is the prototype, were previously found to share four strongly conserved residues, including an active site tyrosine directly involved in transesterification. However, few ad...
متن کاملGenome engineering with custom recombinases.
Site-specific recombinases are valuable tools for myriad basic research and genome engineering applications. In particular, hybrid recombinases consisting of catalytic domains from the resolvase/invertase family of serine recombinases fused to Cys2-His2 zinc-finger or TAL effector DNA-binding domains are capable of introducing targeted modifications into mammalian cells. Due to their inherent m...
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ژورنال
عنوان ژورنال: FEMS Microbiology Reviews
سال: 2021
ISSN: 1574-6976
DOI: 10.1093/femsre/fuab004